OSMT (Ontario Society of Medical Technologists) Practice Exam

For decalcifying bone, what is the recommended volume of fluid used per volume of tissue?

1. a. 10x

2. b. 20x

3. c. 70x

4. d. 200x

The correct answer is: b. 20x

The recommended volume of fluid used for decalcifying bone is often cited as 10 to 20 times the volume of the tissue to ensure effective decalcification. The rationale for using this volume is to provide adequate buffering and ensure that the decalcifying agent can penetrate and act throughout the entire tissue sample uniformly. Using 20 times the volume of tissue strikes a balance between efficiency in the decalcification process while minimizing any potential negative effects on tissue morphology. When volumes exceed this, there may be diminishing returns in terms of the effectiveness of decalcification, as the proportion of decalcifying agent to the bone tissue may become less effective due to an over-dilution. Choosing a smaller volume, such as 10 times, might not be as effective in ensuring complete decalcification, particularly in denser bone samples. Thus, using 20 times the volume provides a reliable method for ensuring thorough decalcification without running into the issues associated with either excessive or insufficient decalcifying fluid.