OSMT (Ontario Society of Medical Technologists) Practice Exam

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Lowenstein Jensen media is suitable for the isolation of which type of organisms?

  1. Enteric organisms

  2. Fungi

  3. Hemophilus

  4. Mycobacteria

The correct answer is: Mycobacteria

Lowenstein Jensen media is specifically designed for the isolation and cultivation of mycobacteria, particularly Mycobacterium tuberculosis. This formulation contains nutrients that are conducive to the growth of these slow-growing bacteria, as well as a high concentration of potato starch which provides a carbon source and helps to inhibit the growth of competing organisms. The slant structure of the medium also allows for a larger surface area, promoting optimal growth conditions for mycobacteria. Lowenstein Jensen media is not suitable for isolating enteric organisms, fungi, or Hemophilus. Enteric organisms typically require a different culture medium that supports their growth and survival in a gastrointestinal environment. Fungi require media that are rich in nutrients tailored to their specific growth requirements. Hemophilus species often need enriched media, such as chocolate agar, which contains factors such as hemin and nicotinamide adenine dinucleotide (NAD) that promote their growth.