OSMT (Ontario Society of Medical Technologists) Practice Exam

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What should the media pH match when prepared?

Require only minor adjustment
Match the pH stated on the product label
Always be neutral
Decrease as it cools

The correct answer is: Match the pH stated on the product label

The media pH should match the pH stated on the product label because this is crucial for ensuring optimal growth conditions for the microorganisms being cultured. Each type of media is formulated with a specific pH that is essential for the biochemical processes necessary for the activity of the media components as well as the growth of the target organisms. Deviations from the recommended pH can lead to suboptimal growth, altered metabolic rates, or even the death of the microorganisms. While some adjustments can be made to the media, it is important that it aligns with the specifications provided by the manufacturer to maintain the intended functionality of the media. Ensuring the correct pH not only helps in achieving reliable results but also maintains the integrity of the research or diagnostic process.