OSMT (Ontario Society of Medical Technologists) Practice Exam

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When is a lake produced in staining?

A mordant combines with a tissue
A dye combines with a tissue
A mordant combines with a stain
A stain combines with a tissue

The correct answer is: A mordant combines with a tissue

A lake in the context of staining refers to a complex formed when a dye (often a colorant) interacts with a mordant, which is a chemical used to enhance the binding of the dye to the tissue. This interaction stabilizes the dye on the tissue, leading to a more vivid and durable color. When a mordant combines with a tissue, it acts as a binding agent that prepares the tissue to attract the dye more effectively. This combination can lead to a more pronounced and stable staining effect. The mordant facilitates a chemical reaction that results in a lake, enhancing the overall visibility of cellular structures under a microscope.