OSMT (Ontario Society of Medical Technologists) Practice Exam

Where should tissue sections with a calcified surface be placed in relation to the knife?

1. a. the knife passes over this part first

2. b. the knife passes over this part last

3. c. calcified surface runs perpendicular to the knife cut

4. d. it doesn't matter as long as the sections are properly fixed

The correct answer is: b. the knife passes over this part last

Placing tissue sections with a calcified surface in such a way that the knife passes over this part last is crucial for achieving clean, accurate cuts. Calcified tissues are typically harder and more brittle than surrounding tissues. When the knife encounters the calcified surface at the end of the cut, it is less likely to cause excessive damage to the knife edge and allows for a cleaner, smoother slicing action. This positioning also helps to avoid any unnecessary pressure that could crush or distort soft tissue sections nearby. In contrast, if the knife were to pass over the calcified area first, it could lead to chipping or dulling of the blade, compromising the quality of the sectioning process. Proper orientation is key to maintaining the integrity of both the knife and the tissue sections, ultimately resulting in better histological outcomes.