OSMT (Ontario Society of Medical Technologists) Practice Exam

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Which of the following is NOT a Romanowsky stain:

a. Leishman
b. Giemsa
c. Jefferson
d. Wright's

The correct answer is: c. Jefferson

The correct answer highlights that Jefferson stain is not classified as a Romanowsky stain. Romanowsky stains are a group of staining techniques that utilize a combination of eosin and methylene blue, commonly used in microscopy to differentiate cellular components and detect abnormalities in blood and other biological samples. Leishman, Giemsa, and Wright's stains are all considered Romanowsky stains as they share the same basic principle and are effective in staining cells for various hematological evaluations. They are favored for their ability to provide detailed morphological features of blood cells, making them invaluable in clinical diagnostics. Jefferson stain, on the other hand, does not fit within this category as it is a different methodology and staining process. By understanding the characteristics of each type of stain and their applications, we can appreciate why Jefferson is distinguished from the other options.